GSM2475078: H9 ESCs TFIIIC ChIP-seq (TFIIIC-110) rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
GTF3C1
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC H9
NA
NA
Attributes by original data submitter
Sample
source_name
H9 ESCs
antibody
TFIIIC Santa Cruz sc-81406
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed using 1% formaldehyde cross-linking for 10 minutes at room temperature. Cells were lysed with 1X PBS in RIPA buffer and sonicated for 30 minutes in a biorupter on high with 50s "on" cycles followed by 10s "off". Chromatin lysate was cleared with a 1 minue high speed spin and incubated with antibody overnight at 4o. Libraries for high-throughput sequencing on Illumina Hi-seq were generated using TruSeq library preparation protocol.